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Document Type |
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Thesis |
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Document Title |
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CYTOGENETIC AND MOLECULAR STUDIES OF GENOTOXICITY AND CARCINOGENICITY OF ALKALOID FRACTION OF RHAZYA STRICTA LEAVES WATER EXTRACT دراسات خلوية وراثية وجزيئية للسمية الوراثية والتأثير المسرطن لجزء القلويدات من المستخلص المائي لأوراق حرمل الرازي |
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Subject |
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Rue (plant) - Physiology |
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Document Language |
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Arabic |
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Abstract |
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Several researches were conducted on alkaloids in order to identify species with medical significance or toxic effects or producing pharmacological effects. This has been the interests of researchers in the field of phytochemistry for more than 150 years. Rhazya stricta is a plant commonly used in the Gulf region and some other Asian regions for many medical purposes. The aim of this study was to evaluate the genotoxicity of alkaloids isolated from the aqueous extract of Rhazya stricta to find out components of this extract responsible for genotoxicity. Alkaloids and non-alkaloids fractions were isolated from the aqueous extract of Rhazya stricta which was collected from Saudi Arabia to evaluate their mutagenic and genotoxic effects. Alkaloids and non-alkaloids fractions were isolated from the aqueous extract of Rhazya stricta which was collected from Saudi Arabia to evaluate their mutagenic and genotoxic effects.
The genotoxicity and carcinogenicity for the alkaloid fraction isolated from the aqueous extract of Rhazya stricta was done molecularly by RAPD and Comet and cytogenetically by micronucleus test. The biochemical assay for Lipids, Glucose and Liver and Kidney functions was detected in adult 105 male wistar rats with three doses 0.11, 0.22 and 0.33 mg/Kg body weight and compared with non-alkaloid fraction isolated from the aqueous extract of Rhazya stricta, and other groups that take three doses 0.1, 0.12, and 0.15 of aqueous whole extract of Rhazya stricta.
The biochemical results showed that there were significant increases in insulin and ALT levels and significant decreases in TG and HOMA-IR insulin resistance levels in the groups treated by Rhazya stricta whole water extract and the groups treated by alkaloid isolated from the aqueous extract of this plant after five months of the treatment. The molecular analysis by RAPD technique for testing the genotoxicity showed that there were significant changes in length and number of DNA bands after the treatment by RAPD primers in the groups treated by Rhazya stricta whole water extract and that treated by alkaloid isolated from the aqueous extract of this plant. The COMET slides showed a significant increase in the total number of tailed cell in the groups treated by Rhazya stricta whole water extract and that treated by alkaloid isolated from the plant aqueous extract. This showed also a necrosis in the cells in both strand of DNA. Results of this study indicated that the alkaloid isolated from the aqueous extract of Rhazya stricta improved lipid blood profiles [cholesterol, high density lipoproteins- cholesterol (HDL-c), low density lipoproteins- cholesterol (LDL-c), triglyceride (TG)] and the functions of liver and kidney. Moreover, this extract had affect in blood sugar, insulin and insulin resistance. However, its effect on DNA appeared after months of the treatment which may indicate its future use in the treatment of metabolic syndrome. The results indicate significantly decrease in cell Mitotic index in all concentrations used in the treated groups by whole water extract (4, 5, and 6 g / kg of body weight) and groups treated by Alkaloids isolated from the aqueous extract of the plant (0.11, and 0.22, and 0.33 g / kg of body weight), and a proportional relationship with the succession of time periods compared to the control group. The results also recorded many chromosomal aberrations such as fractures and gaps and viscosity. The microscopic examination revealed the prevalence of mass death of the cells Necrosis at all concentrations used, this can be benefit from the application of this property to the cancer cells (anti-cancer effect). However, such use should be under control. Moreover, alkaloids fractions should be identified and tested separately to determine the component of alkaloids presented in the water extract which are responsible for its mutagenic effect. Integration of more than one component for synergistic effect is also possible either from different alkaloids or from mixture of alkaloid or non-alkaloid components. Further researches are needed to evaluate the relationship between alkaloids of Rhazya stricta and fat and carbohydrate metabolisms as well as the genotoxicity and carcinogenicity of this plant. |
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Supervisor |
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Nabih A. Baeshin |
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Thesis Type |
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Doctorate Thesis |
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Publishing Year |
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1433 AH
2012 AD |
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Number Of Pages |
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168 |
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Co-Supervisor |
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. Hasen M. al Ashmaoi |
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Added Date |
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Wednesday, February 26, 2014 |
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Researchers
| سونهام سامي يغمور | Yaghmoor, Soonham Sami | Investigator | Doctorate | |
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